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Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

DOI :

10.3791/58933-v

February 15th, 2019

February 15th, 2019

7,354 Views

1Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Research Center, 2Department of Medicine, University of Washington, 3Department of Pathology, University of Washington

The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.

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Preparation

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