Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

10.5K Views

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10:29 min

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August 14th, 2019

DOI :

10.3791/59873-v

August 14th, 2019

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Jessica Garcia¹^,²^,³, David Barthelemy¹^,²^,³, Florence Geiguer¹^,², Julie Ballandier¹^,²^,³, Kathryn W. Li⁴, Jean-Phillippe Aurel⁴, Frédérique Le Breton⁵, Claire Rodriguez-Lafrasse¹, Brigitte Manship³, Sébastien Couraud⁶^,⁷, Léa Payen¹^,²^,³

¹Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, ²Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, ³University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, ⁴Biolidics Limited, ⁵Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, ⁶Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, ⁷EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University

Summary

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PD L1 Characterization

Circulating Tumor Cells

Non small Cell Lung Cancer

Immunofluorescence

Microfluidic Technology

CTC Extraction

Cytological Analysis

Malignant Cytomorphological Characteristics

Genetic Aberrations

FISH

Transcriptomic Analysis

Pre analytical Enrichment

Potassium EDTA Tube

Red Blood Cell Lysis Buffer

Cell Resuspension

Chapters in this video

0:04

Title

1:11

Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples

3:10

Spiral Microfluidic Device CTC Enrichment

4:20

Immunofluorescence (IF) Staining

6:32

IF Image Acquisition and Analysis

8:07

Results: Representative CTC Characterization and Enumeration

9:56

Conclusion

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