JoVE Logo
Faculty Resource Center

Sign In

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System Video (Video) | JoVE

DOI :

10.3791/59962-v

July 31st, 2019

July 31st, 2019

8,705 Views

1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Here, we describe a protocol to introduce a gene knockout into the extracellular amastigote of Trypanosoma cruzi, using the CRISPR/Cas9 system. The growth phenotype can be followed up either by cell counting of axenic amastigote culture or by proliferation of intracellular amastigotes after host cell invasion.

-- Views

Related Videos

article

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format Video (Video) | JoVE

article

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells Video (Video) | JoVE

article

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing Video (Video) | JoVE

article

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells Video (Video) | JoVE

article

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio Video (Video) | JoVE

article

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9 Video (Video) | JoVE

article

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Video (Video) | JoVE

article

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation Video (Video) | JoVE

article

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells Video (Video) | JoVE

article

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells Video (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved