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Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

DOI :

10.3791/59962-v

July 31st, 2019

July 31st, 2019

8,681 Views

1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Here, we describe a protocol to introduce a gene knockout into the extracellular amastigote of Trypanosoma cruzi, using the CRISPR/Cas9 system. The growth phenotype can be followed up either by cell counting of axenic amastigote culture or by proliferation of intracellular amastigotes after host cell invasion.

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