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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

DOI :

10.3791/60085-v

September 25th, 2019

September 25th, 2019

7,883 Views

1Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, University of Pennsylvania

This protocol provides a method to facilitate the generation of defined heterozygous or homozygous nucleotide changes using CRISPR-CAS9 in human pluripotent stem cells.

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