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DOI :
10.3791/60465-v
•
8:02 min
February 19th, 2020
Chapters
0:05
Introduction
0:41
Sample Preparation
1:41
Bisulfate Conversion
2:20
CD8+ T Cell and Regulatory T Cell (Treg) Assays
5:28
T Helper Cell 17 (Th17) Assay
6:37
Results: Representative Flow and Methylation Assay Data Comparisons
7:30
Conclusion
在这里,我们描述了一种通过使用定量PCR(qPCR)检测的表观遗传特征来确定免疫细胞特性和纯度的可靠方法。特定位点的脱甲基化作为特定细胞类型的唯一标识符,允许识别CD8+、调节或Th17 T细胞。
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