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DOI :
10.3791/60465-v
•
8:02 min
February 19th, 2020
Chapters
0:05
Introduction
0:41
Sample Preparation
1:41
Bisulfate Conversion
2:20
CD8+ T Cell and Regulatory T Cell (Treg) Assays
5:28
T Helper Cell 17 (Th17) Assay
6:37
Results: Representative Flow and Methylation Assay Data Comparisons
7:30
Conclusion
ここでは、定量PCR(qPCR)を用いて検出されたエピジェネティックシグネチャを通じて免疫細胞の同一性と純度を決定する堅牢な方法について説明します。特定の遺伝子座でのDNA脱メチル化は、特定の細胞タイプの一意の識別子として機能し、CD8+、調節、またはTh17 T細胞の識別を可能にします。
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