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Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

DOI :

10.3791/60736-v

13:48 min

January 18th, 2020

January 18th, 2020

7,047 Views

1Vaccine and Infectious Disease Organization-International Vaccine Centre, 2Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, 3Department of Cell Biology and Genetics, Shenzhen University Health Science Center

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a method used to establish interactions between transcription factors and the genomic sequences they control. This protocol outlines techniques for performing ChIP-seq with bacterial biofilms, using Salmonella enterica serovar Typhimurium bacterial biofilm as an example.

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ChIP seq

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