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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

DOI :

10.3791/56085-v

10:05 min

December 12th, 2017

December 12th, 2017

21,354 Views

1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency

We present a modified native chromatin immunoprecipitation sequencing (ChIP-seq) methodology for the generation of sequence datasets suitable for a nucleosome density ChIP-seq analytical framework integrating micrococcal nuclease (MNase) accessibility with histone modification measurements.

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Chromatin Immunoprecipitation Sequencing Libraries

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