JoVE Logo
Faculty Resource Center

Sign In

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

DOI :

10.3791/59375-v

May 10th, 2019

May 10th, 2019

13,121 Views

1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

Chromatin immunoprecipitation (ChIP) is a powerful tool for understanding the molecular mechanisms of gene regulation. However, the method involves difficulties in obtaining reproducible chromatin fragmentation by mechanical shearing. Here, we provide an improved protocol for a ChIP assay using enzymatic digestion.

-- Views

Related Videos

article

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

article

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

article

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

article

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

article

Author Spotlight: Efficient CRISPR/Cas9 Genome Editing in Bone Marrow-Derived Macrophages for Precise Gene Disruption

article

Author Spotlight: PGC Cryopreservation – An Alternative Approach Towards Long-Term Preservation of Drosophila Strains

article

Collection of F1 Generation Caenorhabditis elegans for Chromatin Immunoprecipitation (ChIP)

article

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

article

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

article

Collection of Primordial Germ Cells from Drosophila Donor Embryos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved