Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

7.5K Views

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08:54 min

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May 19th, 2020

DOI :

10.3791/60861-v

May 19th, 2020

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Andrew Reckers¹, Anita Wen¹, David Aguilar¹, Peter Bacchetti², Monica Gandhi³, John Metcalfe⁴, Roy Gerona¹

¹Department of Obstetrics, Gynecology and Reproductive Sciences, TB Hair Analysis Laboratory, University of California San Francisco, ²Department of Epidemiology and Biostatistics, University of California San Francisco, ³Department of Medicine, Division of HIV, Infectious Diseases, and Global Medicine, University of California San Francisco, ⁴Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of California San Francisco

Summary

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Validated LC MS MS Panel

Drug resistant TB

Drug Metabolism

Patient Adherence

Drug Levels Measurement

Treatment Regimen

Hair Samples

Analyte Mass Spectrometry

Drug Concentration

Sample Extraction

Homogenization Procedure

Treatment Plan

Chapters in this video

0:04

Introduction

1:20

Hair Sampling

2:32

Drug Extraction

4:18

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) Preparation

5:35

Data Analysis

6:47

Results: Representative Drug-Resistant (DR) TB Medication Quantification

7:55

Conclusion

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