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Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

DOI :

10.3791/61030-v

6:01 min

July 3rd, 2020

July 3rd, 2020

7,881 Views

1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology

Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.

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Standardized Methods

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