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Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

DOI :

10.3791/57938-v

8:33 min

July 24th, 2018

July 24th, 2018

10,557 Views

1Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University, 2Institute of Biomedical Studies, Baylor University

Here, we present a protocol to examine larval zebrafish and fathead minnow locomotor activities and photomotor responses (PMR) using an automated tracking software. When incorporated in common toxicology bioassays, analyses of these behaviors provide a diagnostic tool to examine chemical bioactivity. This protocol is described using caffeine, a model neurostimulant.

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Experimental Protocol

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