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Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes

DOI :

10.3791/61061-v

August 27th, 2020

August 27th, 2020

3,856 Views

1CNRS, Institut Jacques Monod, Université de Paris, F 75006, Paris, France

We present here an improved protocol for the whole-mount preparation and immunostaining of Drosophila testes, suitable for confocal microscopy and also allowing reproducible and reliable labeling. We illustrate this protocol by 3D representation and quantification of colocalization experiments on the large S4-S5 spermatocytes.

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Immunostaining

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