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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

DOI :

10.3791/51604-v

6:36 min

July 17th, 2014

July 17th, 2014

23,699 Views

1Department of Biological Sciences, University of Notre Dame

The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.

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Keywords Zebrafish Embryo

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