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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

DOI :

10.3791/51060-v

8:20 min

December 20th, 2013

December 20th, 2013

19,193 Views

1Department of Oncological Sciences, University of Utah

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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Keywords Zebrafish

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