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Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

DOI :

10.3791/61122-v

May 12th, 2020

May 12th, 2020

22,624 Views

1School of Molecular Sciences, The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University

The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

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