JoVE Logo
Faculty Resource Center

Sign In

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

DOI :

10.3791/59063-v

March 6th, 2019

March 6th, 2019

8,964 Views

1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.

Tags

Synthesis

-- Views

Related Videos

article

Synthesis of Hypervalent Iodonium Alkynyl Triflates for the Application of Generating Cyanocarbenes

article

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

article

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

article

Protocol for the Synthesis of Ortho-trifluoromethoxylated Aniline Derivatives

article

Synthesis and Characterization of Supramolecular Colloids

article

HKUST-1 as a Heterogeneous Catalyst for the Synthesis of Vanillin

article

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

article

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst

article

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

article

Chemical Precipitation Method for the Synthesis of Nb2O5 Modified Bulk Nickel Catalysts with High Specific Surface Area

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved