Representative Images of Larval and Adult Optic Lobes
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Conclusion
Transcript
We describe a protocol to dissect, immunostain and mount larval and adult Drosophila brains with a special emphasis on the optic lobes. Given the optic lobe's complex three-dimensional organization, it is anticipated that the protocol described he
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This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures.Â