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11:20 min
May 12th, 2020
DOI :
10.3791/61275-v
Chapters
0:04
Introduction
0:48
Phage Stock and Phage Cocktail Preparation
3:45
Zebrafish Embryo Cystic Fibrosis Transmembrane Conductance Regulator (cftr) Morpholino Microinjection
4:51
Zebrafish Embryo P. aeruginosa (PAO1) Bacteria and Phage Cocktail Microinjection
6:12
Time-Lapse Imaging Preparation
7:06
Bacterial Burden and PAO1 Infection Embryonic Lethality Evaluation
8:48
Results: Representative Phage Therapy Effects on Pseudomonas aeruginosa Infection in Cystic Fibrosis (CF) Zebrafish Embryos
10:36
Conclusion
Transcript
该协议有助于制备和管理病毒和噬菌体鸡尾酒,以对抗斑马鱼胚胎中的伪单体亚鲁吉诺萨。这种技术的主要优点是,它允许在体内评估噬菌体在对抗细菌感染的功效。噬菌体鸡尾酒在野生型和囊性纤维化斑马鱼模型中都非常有效。
为囊性纤维化患者的细菌感染提供噬菌体治疗的可能性。噬菌体疗法也可以用于抵消其他模型系统中的特定细菌感染。为实验准备噬菌的植物。
将 1.25 倍 10 添加到第七噬菌体,加入 0.05 P.aeruginosa 培养的 OD 600,将 1 倍 10 的感染添加到负
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Summary
这里介绍的是伪 多多多酸菌感染和 噬菌体治疗应用囊性纤维化(CF)斑马鱼胚胎的协议。
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噬菌体纤维化 斑马鱼胚 胎中伪多多多阿韦诺萨感染的噬菌体治疗应用
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