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Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

DOI :

10.3791/61297-v

May 15th, 2020

May 15th, 2020

3,260 Views

1Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

The goal of this protocol is to inject Rhodamine-conjugated globular actin into Drosophila embryos and image intranuclear actin rod assembly following heat stress.

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