Isolation of Porcine Cumulus-oocyte Complexes (COCs)
2:35
Encapsulation in Fibrin-alginate Hydrogel Beads
4:48
Encapsulation in Super-hydrophobic Fluorinated Ethylene Propylene (FEP) Microbioreactors
6:17
Results: Morphology and Viability of COCs after Encapsulation
7:15
Conclusion
Transcript
This protocol for encapsulation of porcine oocytes makes it possible to maintain spherical organization of COCs. This prevents their flattening and consequent disruption of gap junctions between the oocyte and surrounding follicular cells. The mai
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Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.