July 30th, 2020
•Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.
Related Videos
Title Cell Encapsulation by Droplets
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
PuraMatrix Encapsulation of Cancer Cells
Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Single Oocyte Bisulfite Mutagenesis
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
FRET Imaging in Three-dimensional Hydrogels
Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved