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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

DOI :

10.3791/53944-v

April 22nd, 2016

16,253 Views

1Department of Chemical Engineering, Konkuk University, 2Department of Chemical Engineering, University of Michigan

Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.

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