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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

DOI :

10.3791/61557-v

February 28th, 2021

February 28th, 2021

5,138 Views

1Department of Biomedical Sciences, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, 2Stem Cell Immunomodulation Research Center, University of Ulsan College of Medicine

People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.

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CRISPR mediated Base Editors

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