Creation of BRCA1 Variants using CRISPR-mediated Base Editing Tools
4:15
Sample Preparation for Illumina Next-Generation Sequencing (NGS)
7:01
Results: Functional Study of BRCA1 using CRISPR-Cas9 Systems
8:34
Conclusion
Transcript
By efficiently inducing point mutations using the CRISPR-mediated cytosine bases editor, the functionality of BRCA1 variants, which is important for disease prevention and diagnosis, can be identified. The advantage of this technique is that it di
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People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.