Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

1.2K Views

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06:08 min

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February 10th, 2023

DOI :

10.3791/64535-v

February 10th, 2023

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Claudia Manriquez Roman¹^,²^,³^,⁴^,⁵, R. Leo Sakemura¹^,², Brooke L. Kimball¹^,², Fang Jin⁶, Roman H. Khadka⁶, Mohamad M. Adada¹^,², Elizabeth L. Siegler¹^,², Aaron J. Johnson⁶, Saad S. Kenderian¹^,²^,⁶

¹T Cell Engineering Laboratory, Mayo Clinic, Rochester, ²Division of Hematology, Mayo Clinic, Rochester, ³Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, ⁴Department of Molecular Medicine, Mayo Clinic, Rochester, ⁵Regenerative Sciences PhD Program, Mayo Clinic, Rochester, ⁶Department of Immunology, Mayo Clinic, Rochester

Summary

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Chimeric Antigen Receptor

T Cell Toxicities

Acute Lymphoblastic Leukemia

Patient derived Xenograft

CAR T Administration

Translatable Model

Tumor Burden Assessment

Chapters in this video

0:04

Introduction

0:49

Assessment of CART19 Cell‐Associated Toxicities

1:33

MRI Imaging

4:02

Results: Assessment of CART Cell‐Associated Toxicities in a PDX Mice Model

5:17

Conclusion

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