University of Ulsan College of Medicine

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Ji-Eun See^1,2, Ha Rim Shin^1,2, Gayoung Jang^1,2, Jiyeon Kweon^1,2, Yongsub Kim^1,2

¹Department of Biomedical Sciences, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, ²Stem Cell Immunomodulation Research Center, University of Ulsan College of Medicine

People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.

Medicine

Robot-Assisted Kidney Transplantation

Seong Jun Lim¹, Youngmin Ko¹, Dong Hyun Kim¹, Joo Hee Jung¹, Hyunwook Kwon¹, Young Hoon Kim¹, Sung Shin¹

¹Division of Kidney and Pancreas Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine

This paper provides technical details for robot-assisted kidney transplantation from a living donor.