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An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

DOI :

10.3791/61648-v

September 15th, 2020

September 15th, 2020

2,953 Views

1Molecular Biology Program, Memorial Sloan Kettering Cancer Center

Tracking individual translation events allows for high-resolution kinetic studies of cap-dependent translation mechanisms. Here we demonstrate an in vitro single-molecule assay based on imaging interactions between fluorescently labeled antibodies and epitope-tagged nascent peptides. This method enables single-molecule characterization of initiation and peptide elongation kinetics during active in vitro cap-dependent translation.

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Keywords Single molecule Imaging

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