Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
3.1K Views
•
06:26 min
•
September 20th, 2020
DOI :
September 20th, 2020
•Ragini M. Mistry1,2, Pankaj K. Singh1,3, Maureen G. Mancini1,2, Fabio Stossi1,2, Michael A. Mancini1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine
Chapters in this video
0:04
Introduction
0:34
RNA Fluorescence In Situ Hybridization (FISH) Fixation
1:14
RNA FISH Hybridization
2:16
Imaging Preparation
3:08
High Resolution Microscopy and Image Processing
4:05
Image Analysis
4:45
Results: Representative Image Segmentation and E2-Induced GREB1 Quantification
5:56
Conclusion
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