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Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

DOI :

10.3791/61660-v

12:38 min

April 11th, 2021

April 11th, 2021

5,938 Views

1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258, Université Bordeaux, 3ELVESYS SAS

This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip.

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Protein Crystallization

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