JoVE Logo
Faculty Resource Center

Sign In

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

DOI :

10.3791/61660-v

12:38 min

April 11th, 2021

April 11th, 2021

6,031 Views

1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258, Université Bordeaux, 3ELVESYS SAS

This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip.

Tags

Protein Crystallization

-- Views

Related Videos

article

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

article

Dynamic Electrochemical Measurement of Chloride Ions

article

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

article

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects

article

Quantifying X-Ray Fluorescence Data Using MAPS

article

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

article

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

article

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

article

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

article

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved