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Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

DOI :

10.3791/52370-v

March 12th, 2015

March 12th, 2015

9,198 Views

1X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, 2Department of Physics and Astronomy, Northwestern University

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

Tags

X ray Fluorescence Imaging

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