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Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

DOI :

10.3791/61868-v

5:52 min

November 25th, 2020

November 25th, 2020

7,406 Views

1Institute for Ophthalmic Research, University of Tübingen

The protocol describes organotypic explants of mouse neuroretina, cultivated together with its retinal pigment epithelium (RPE), in R16 defined medium, free of serum and antibiotics. This method is relatively simple to perform, less expensive, and time-consuming when compared to in vivo experiments, and can be adapted to numerous experimental applications.

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Long term

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