JoVE Logo
Faculty Resource Center

Sign In

Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

DOI :

10.3791/61868-v

5:52 min

November 25th, 2020

November 25th, 2020

6,657 Views

1Institute for Ophthalmic Research, University of Tübingen

The protocol describes organotypic explants of mouse neuroretina, cultivated together with its retinal pigment epithelium (RPE), in R16 defined medium, free of serum and antibiotics. This method is relatively simple to perform, less expensive, and time-consuming when compared to in vivo experiments, and can be adapted to numerous experimental applications.

Tags

Long term

-- Views

Related Videos

article

Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

article

Methanol-Based Whole-Mount Preparation for the Investigation of Retinal Ganglion Cells

article

Author Spotlight: A Unique Mouse Model of Asphyxia-Induced Cardiac Arrest

article

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus

article

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

article

Ultrasound-Guided Needle Release Combined with Corticosteroid Injection for the Treatment of Supinator Syndrome

article

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

article

Low-Cost Electroencephalographic Recording System Combined with a Millimeter-Sized Coil to Transcranially Stimulate the Mouse Brain In Vivo

article

A Procedure for Mouse Dorsal Root Ganglion Cryosectioning

article

Author Spotlight: An Accurate and Quantitative Approach to Study Visual Feature Selectivity of the Optokinetic Reflex in Mice

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved