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Long-term Time Lapse Imaging of Mouse Cochlear Explants

DOI :

10.3791/52101-v

November 2nd, 2014

November 2nd, 2014

9,342 Views

1Biological Sciences Platform, Sunnybrook Research Institute, 2Department of Otolaryngology - Head and Neck Surgery, University of Toronto, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

Live imaging of the embryonic mammalian cochlea is challenging because the developmental processes at hand operate on a temporal gradient over ten days. Here we present a method for culturing and then imaging embryonic cochlear explant tissue taken from a fluorescent reporter mouse over five days.

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Long term Time lapse Imaging

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