JoVE Logo
Faculty Resource Center

Sign In

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

DOI :

10.3791/3742-v

March 7th, 2012

March 7th, 2012

26,262 Views

1Department of Physics, University of California, San Diego, 2Department of Engineering Science and Mechanics, Pennsylvania State University , 3Department of Neurosurgery, Pennsylvania State University , 4Section of Neurobiology, University of California, San Diego

We present a method to form an imaging window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This method is well suited for longitudinal studies of blood flow, cellular dynamics, and cell/vascular structure using two-photon microscopy.

Tags

Polished And Reinforced Thinned skull Window

-- Views

Related Videos

article

Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation

article

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

article

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

article

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear

article

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

article

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

article

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

article

Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

article

Long-term Time Lapse Imaging of Mouse Cochlear Explants

article

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved