Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
4.1K Views
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08:39 min
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December 22nd, 2020
DOI :
December 22nd, 2020
•1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Goettingen, Germany
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Chapters in this video
0:05
Introduction
0:45
Cell Preparation
1:35
Microscope Setup
3:28
Voltage Sensitive Dye Cell Loading
4:24
Electrical Field Stimulation
4:59
Optical Action Potential (AP) Acquisition
6:01
Data Analysis
6:55
Results: Representative Optical AP Profiles of Isolated Native Murine and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
7:59
Conclusion
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