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JoVE Journal

Bioengineering

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Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

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08:39 min

December 22nd, 2020

December 22nd, 2020

3,951 Views

0:05

Introduction

0:45

Cell Preparation

1:35

Microscope Setup

3:28

Voltage Sensitive Dye Cell Loading

4:24

Electrical Field Stimulation

4:59

Optical Action Potential (AP) Acquisition

6:01

Data Analysis

6:55

Results: Representative Optical AP Profiles of Isolated Native Murine and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

7:59

Conclusion

Transcript

This protocol can provide high throughput measurements of cellular electrophysiology in a manner that befits the rapidly developing technology of induced pluripotent stem cell-derived cardiomyocytes. The main advantage of this technique is that it

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Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system.

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