Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

5.5K Views

09:46 min

January 20th, 2023

DOI :

10.3791/64715-v

January 20th, 2023


Explore More Videos

Blood Vessel Organoids

Chapters in this video

0:04

Introduction

0:52

Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension

2:34

Day 1: Mesoderm Induction of the Aggregates

3:41

Day 4: Vascular Induction and Priming of the Aggregates

4:20

Day 6: Aggregate Embedding and Vessel Sprout Induction

6:02

Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)

7:25

Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs

9:13

Conclusion

Related Videos

article

09:02

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

16.7K Views

article

10:03

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

9.4K Views

article

09:16

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

9.9K Views

article

10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

10.2K Views

article

10:40

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

10.4K Views

article

08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

4.4K Views

article

08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

4.0K Views

article

09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

3.2K Views

article

10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

2.9K Views

article

09:23

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

3.2K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved