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Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

2.5K Views

10:00 min

December 11th, 2020

DOI :

10.3791/61957-v

December 11th, 2020


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Oxidative Stress

Chapters in this video

0:04

Introduction

0:52

Conditioned Medium Preparation and Collection

2:47

Treatment of RPE Cells with Growth Factors and H2O2

4:33

Glutathione Assay

6:00

Cytotoxicity Assay and Microscopic Analysis

7:24

Results: Representative Oxidative Stress Induction and Analysis

9:10

Conclusion

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