JoVE Logo
Faculty Resource Center

Sign In

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

DOI :

10.3791/62008-v

9:08 min

December 22nd, 2020

December 22nd, 2020

5,223 Views

1The Graduate Center - Advanced Science Research Center, Neuroscience Initiative, The City University of New York, 2The City College of New York, CUNY, 3The Bronx High School of Science, 4The Graduate Center - Advanced Science Research Center, Structural Biology Initiative, The City University of New York, 5The Graduate Center - Advanced Science Research Center, MALDI MS Imaging Joint Core Facility, The City University of New York

The goal of this protocol is to provide detailed guidance on the sample preparation when planning for experiments using MALDI MSI to maximize metabolic and molecular detection in biological samples.

Tags

Sample Preparation

-- Views

Related Videos

article

MALDI Sample Preparation: the Ultra Thin Layer Method

article

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

article

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

article

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

article

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

article

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

article

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

article

Sample Preparation for Mass Cytometry Analysis

article

Cellular Redox Profiling Using High-content Microscopy

article

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved