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Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

DOI :

10.3791/56482-v

January 4th, 2018

January 4th, 2018

36,813 Views

1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences, Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network, Korea Brain Research Institute

This protocol describes the various techniques necessary for transmission electron microscopy including negative staining, ultrathin sectioning for detailed structure, and immuno-gold labelling to determine the positions of specific proteins in exosomes.

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Sample Preparation

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