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A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

DOI :

10.3791/4083-v

14:44 min

September 24th, 2012

September 24th, 2012

20,080 Views

1Max Planck Institute of Molecular Plant Physiology, 2University of Kaiserslautern

We present a variation of the QUICK (QUantitative Immunoprecipitation Combined with Knockdown) approach that was introduced previously to distinguish between true and false protein-protein interactions. Our approach is based on 15N metabolic labeling, the modulation of affinities of protein-protein interactions by the presence/absence of ATP, immunoprecipitation, and quantitative mass spectrometry.

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Protein protein Interactions

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