Deuterium (D2O) Incorporation Treatment in the Presence of Antibiotics
3:50
Preparation of Bacteria in Urine Environment and Blood Environment
5:42
Stimulated Raman Scattering (SRS) Imaging of D2O Metabolic Incorporation in a Single Bacterium
8:44
Results: The Influence of Deuterium Incorporation on Bacterial Growth
11:14
Conclusion
Transcript
Rapid antimicrobial susceptibility testing can be obtained within 2.5 hours in urine and blood, which is considered a tremendous reduction in analysis time compared to the conventional broth microdilution method. It can monitor bacterial metabolic
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This protocol presents rapid antimicrobial susceptibility testing (AST) assay within 2.5 h by single-cell-stimulated Raman scattering imaging of D2O metabolism. This method applies to bacteria in the urine or whole blood environment, which is transformative for rapid single-cell phenotypic AST in the clinic.