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Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

DOI :

10.3791/62622-v

11:51 min

September 22nd, 2021

September 22nd, 2021

6,919 Views

1Cell Therapy Facility, Moffitt Cancer Center and Research Institute, 2Department of Immunology, Moffitt Cancer Center and Research Institute

Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.

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Gamma Delta T Cells

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