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Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

DOI :

10.3791/61470-v

9:08 min

October 14th, 2021

October 14th, 2021

3,994 Views

1Department of Dermatology, University of Arkansas for Medical Sciences

We present a simple protocol for the isolation of peripheral blood mononuclear cells from whole blood obtained from patients diagnosed with Sézary Syndrome, followed by selection of CD4+ T cells, their stimulation with phorbol12-myristate13-acetate and A23187 ionophore, and preparation of RNA for transcriptomic profiling.

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Isolating

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