JoVE Journal

Medicine

This content is Open Access.

11:20

live

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

3.6K Views

•

11:20 min

•

June 14th, 2021

DOI :

10.3791/62638-v

June 14th, 2021

•

Ivan Seah*¹^,², Zengping Liu*¹^,³^,⁴, Daniel Soo Lin Wong¹, Wendy Wong², Graham E. Holder¹^,²^,⁵, Veluchamy Amutha Barathi¹^,⁴^,⁶, Gopal Lingam¹^,²^,⁴, Xinyi Su¹^,²^,³^,⁴, Boris V. Stanzel¹^,⁷^,⁸

¹Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, ²Department of Ophthalmology, National University Hospital, Singapore, ³Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), ⁴Singapore Eye Research Institute (SERI), ⁵UCL Institute of Ophthalmology, ⁶Academic Clinical Program in Ophthalmology, Duke-NUS Medical School, ⁷Macula Center Saar, Eye Clinic Sulzbach, Knappschaft Hospital Saar, ⁸Department of Ophthalmology, University of Bonn

Summary

The non-human primate (NHP) is an ideal model for studying human retinal cellular therapeutics due to the anatomical and genetic similarities. This manuscript describes a method for submacular transplantation of retinal pigment epithelial cells in the NHP eye and strategies to prevent intraoperative complications associated with macular manipulation.

Explore More Videos

Retinal Pigment Epithelium

Transplantation

Non human Primate Model

Degenerative Retinal Diseases

Visual Outcomes

Intravitreal Plasminogen Pretreatment

OCT guided Retinal Bleb Formation

Perfluorocarbon Liquid Vitreous Tamponade

Dual Bore Cannula

Surgical Technique

Posterior Vitreous Detachment

Chapters in this video

0:04

Introduction

1:22

Vitrectomy

4:13

Microscope-Integrated Optical Coherence Tomography (miOCT)-Guided Foveal Detachment

5:49

Removal of Native Retinal Pigment Epithelium

6:21

miOCT-Guided Graft Implantation, Position Adjustment, and Subretinal Fluid Drainage

8:33

Results: Assessment of the Retinal Pigment Epithelial Graft

10:33

Conclusion

Related Videos

article

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

19.6K Views

article

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

17.2K Views

article

Techniques for Processing Eyes Implanted with a Retinal Prosthesis for Localized Histopathological Analysis: Part 2 Epiretinal Implants with Retinal Tacks

11.4K Views

article

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

11.3K Views

article

A Step by Step Protocol for Subretinal Surgery in Rabbits

15.3K Views

article

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

7.1K Views

article

A Surgical Procedure for the Administration of Drugs to the Inner Ear in a Non-Human Primate Common Marmoset (Callithrix jacchus)

8.5K Views

article

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

8.1K Views

article

Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells

2.5K Views

article

Retinal Pathophysiological Evaluation in a Rat Model

4.3K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More