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DOI :
10.3791/62868-v
•
12:24 min
September 30th, 2021
Chapters
0:05
Introduction
1:20
Cell Preparation
2:17
Cell Collection and Counting
4:14
Collection of Fibroblast Conditioned Media
5:17
Heterotypic Tumor/Stromal Spheroids
6:29
Homotypic Hep3B Spheroids in LX2 Conditioned Media
8:18
Calculation of Spheroid Volume
9:17
Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model
11:28
Conclusion
관련 인간 질병을 충실하게 재구성하는 포괄적인 체외 모델은 부족합니다. 현재 연구는 인간 간세포 암에서 종양 기질 상호 작용을 연구하는 신뢰할 수있는 체외 도구인 3차원 (3D) 종양 스페로이드 생성 및 문화를 제시합니다.
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