Mice Preparation for Ultrasound-Guided Injection (US-GI)
2:23
Injection of PANC1 Cells in the Pancreas by Ultrasound-Guided Injection (US-GI) Method
4:17
Results: Development of Orthotopic Pancreatic Cancer (PCa) Xenograft in the Mouse Pancreas
5:06
Conclusion
Transcript
The protocol describes a method to generate an orthotopic mouse model of pancreatic cancer using a system that combines the injection of cells and the evaluation of engraftment and development of tumors. This technique has great reproducibility, i
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We present a protocol to generate a minimally invasive orthotopic pancreatic cancer model by ultrasound-guided injection of human pancreatic cancer cells and the subsequent monitoring of tumor growth in vivo by ultrasound imaging.