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Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

DOI :

10.3791/55357-v

July 18th, 2017

July 18th, 2017

9,548 Views

1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

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Isolation

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