Ex Utero Roller Culture of Embryos from Embryonic Day 7.5 to 11
3:48
Extended Embryo Culture from Embryonic Day 5.5/6.5 to 11
4:38
Results: Continuous and Robust Ex Utero Development of Post Implantation Mouse Embryos
6:34
Conclusion
Transcript
This method will be fundamental for understanding gastrulation along a formation in mammals. By allowing experimentation in living embryos during embryonic stages that have long been hidden inside the maternal uterus. It allows, continues efficien
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An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.