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Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

DOI :

10.3791/63160-v

October 19th, 2021

October 19th, 2021

5,411 Views

1Department of Molecular Genetics, Weizmann Institute of Science

An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.

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