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3.2K Views
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07:36 min
November 15th, 2021
DOI :
10.3791/63282-v
Chapters
0:04
Introduction
1:04
Multiphoton Fluorescence Lifetime Imaging (FLIM) of Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) and Flavin Adenine Dinucleotide (FAD)
2:13
Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) Imaging
3:35
Flavin Adenine Dinucleotide (FAD) Imaging
4:45
Cyanide Experiment Preparation
5:28
Results: The Fluorescence Imaging of MCF-7 Cells Before and After Cyanide Treatment
7:05
Conclusion
Transcript
ADH和FAD是内源性分子,在不同的激发和发射波长下表现出自发荧光。由于它们被用作代谢反应的辅酶,自发荧光显微镜可以评估细胞代谢。NADH和FAD的自发荧光成像不需要外源性标记,是一种具有亚细胞分辨率的非破坏性方法。
自发荧光显微镜可用于活体样品和时间过程研究。自发荧光成像可以广泛应用于任何活细胞或组织。自发荧光成像已用于神经元,癌细胞,肿瘤,体内,干细胞和免疫细胞。
演示该程序的将是德克萨斯A&M大学定量光学成像实验室的研究生Linghao Hu。通过打开多光子荧
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Summary
该协议描述了内源性代谢辅酶,还原烟酰胺腺嘌呤(磷酸盐)二核苷酸(NAD(P)H)和氧化黄素腺嘌呤二核苷酸(FAD)的荧光成像和分析。NAD(P)H 和 FAD 的自发荧光成像提供了一种无标记、无损的方法来评估细胞代谢。
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