Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
6.6K Views
•
14:25 min
•
May 13th, 2022
DOI :
May 13th, 2022
•María V. Vaglienti1,2, Paula V. Subirada1,3, Pablo F. Barcelona1,2, Gustavo Bonacci1,2, María C. Sanchez1,2
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Explore More Videos
Chapters in this video
0:00
Introduction
1:37
Performing the Assay
4:21
Cells Preparaion for Flow Cytometry
6:57
Data Acquisition in a Flow Cytometer
9:00
Analysis of the Acquired Data
12:07
Representative Results
13:37
Conclusion
Related Videos
09:53
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
14.4K Views
04:53
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
30.2K Views
06:10
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
7.2K Views
07:19
Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
9.6K Views
13:20
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
15.8K Views
07:10
Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia
1.7K Views
07:41
Quantification of Microglial Engulfment of Synaptic Material Using Flow Cytometry
1.1K Views
09:08
Detecting Reactive Oxygen Species
31.9K Views
02:00
Fluorescent Probe Imaging Assay: A Technique to Visualize Oxidative Stress in the Reactive Oxygen Species Inducer-Treated Cultured Intestinal Organoid Cells
192 Views
01:52
Flow Cytometry Assay to Quantify Oxidative Stress: An Assay to Quantify Reactive Oxygen Species in Intestinal Organoids Using Fluorogenic Probes
367 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved