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JoVE Journal

Biochemistry

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Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

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06:13 min

March 31st, 2022

March 31st, 2022

2,866 Views

0:05

Introduction

1:08

Incubation with Debranching Enzymes and Reductive Amination

2:37

FACE Analysis

3:32

Data Processing

4:08

Results: Determination of the Average Chain Length of Glycogen

5:42

Conclusion

Transcript

In the present protocol, the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) technique is used to determine the chain length distribution (CLD) and the average chain length (ACL) of glycogen.

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